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ENO1 antibody (C-Term)

Name: ENO1 antibody (C-Term)
SKU: ABIN1882077
Price: 429 USD
Availability: InStock

ENO1 Reactivity: Human, Mouse WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB19995 unconjugated

ENOA Antibody (C-term) (ABIN1882077 and ABIN2839495) western blot analysis in Jurkat,MCF-7, cell line and mouse brain tissue lysates (35 μg/lane). (ENO1 antibody (C-Term))

ENOA Antibody (C-term) (ABIN1882077 and ABIN2839495) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. (ENO1 antibody (C-Term))

IHC (p)

Confocal immunofluorescent analysis of ENOA Antibody (C-term) (ABIN1882077 and ABIN2839495) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). (ENO1 antibody (C-Term))

ENOA Antibody (C-term) (ABIN1882077 and ABIN2839495) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram). (ENO1 antibody (C-Term))

FACS

4 images

(2 references)

Catalog No. ABIN1882077

Datasheet as PDF

Target See all ENO1 Antibodies

ENO1 (Enolase 1 (ENO1))
Binding Specificity
15

7

6

6

4

2

1

1

1

1

1

1

1

1

1

1

1
AA 405-434, C-Term
Reactivity
84

49

31

15

11

6

6

6

4

4

3

3

3

2

1

1

1

1
Human, Mouse
Host
83

15

3
Rabbit
Compatible Secondaries
Clonality
76

24
Polyclonal
Conjugate
55

12

8

5

3

3

3

3

3

3

1

1

1
This ENO1 antibody is un-conjugated
Application
68

34

30

25

19

12

10

9

5

4

2

2

2

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Predicted Reactivity

Pr

Purification

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen

This ENOA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 405-434 amino acids from the C-terminal region of human ENOA.

Clone

RB19995

Isotype

Ig Fraction

Top Product

Discover our top product ENO1 Primary Antibody
anti-Enolase 1 (ENO1) (AA 1-434) antibody
ENO1 Reactivity: Human WB, ELISA, IF, IP, IHC (p) Host: Mouse Monoclonal 8G8 unconjugated

anti-Enolase 1 (ENO1) (AA 2-434) antibody
ENO1 Reactivity: Human WB, ELISA, IHC, IF, IP, FACS Host: Mouse Monoclonal 8H9G12 unconjugated

anti-Enolase 1 (ENO1) (AA 1-434) antibody
ENO1 Reactivity: Human WB, ELISA Host: Mouse Polyclonal unconjugated

anti-Enolase 1 (ENO1) antibody
ENO1 Reactivity: Human, Mouse, Rat IF Host: Rabbit Polyclonal unconjugated

anti-Enolase 1 (ENO1) (AA 1-434) antibody
ENO1 Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Enolase 1 (ENO1) (Middle Region) antibody
ENO1 Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Rabbit, Guinea Pig, Horse, Dog, Pig WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Enolase 1 (ENO1) (AA 1-434) antibody
ENO1 Reactivity: Human WB, ELISA Host: Mouse Monoclonal 8G3 unconjugated

anti-Enolase 1 (ENO1) antibody
KO Validated ENO1 Reactivity: Human WB, IF, ICC, IHC (p), DB, IHC (wm) Host: Rabbit Polyclonal unconjugated

anti-Enolase 1 (ENO1) (AA 246-295) antibody
ENO1 Reactivity: Human, Horse, Pig, Monkey, Chicken WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Enolase 1 (ENO1) (AA 2-428) antibody
ENO1 Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

Application Notes

IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions

For Research Use only
Format

Liquid

Buffer

Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C,-20 °C

Expiry Date

6 months
Cappello, Tomaino, Chiarle, Ceruti, Novarino, Castagnoli, Migliorini, Perconti, Giallongo, Milella, Monsurrò, Barbi, Scarpa, Nisticò, Giovarelli, Novelli: "An integrated humoral and cellular response is elicited in pancreatic cancer by alpha-enolase, a novel pancreatic ductal adenocarcinoma-associated antigen." in: International journal of cancer. Journal international du cancer, Vol. 125, Issue 3, pp. 639-48, (2009) (PubMed).

Wygrecka, Marsh, Morty, Henneke, Guenther, Lohmeyer, Markart, Preissner: "Enolase-1 promotes plasminogen-mediated recruitment of monocytes to the acutely inflamed lung." in: Blood, Vol. 113, Issue 22, pp. 5588-98, (2009) (PubMed).
Target

ENO1 (Enolase 1 (ENO1))

Abstract

ENO1 Products

Synonyms

enolase 1 antibody, F2P9.10 antibody, F2P9_10 antibody, Pgh1 antibody, pENO1 antibody, enolase 1 antibody, phosphopyruvate hydratase antibody, ENO1 antibody, eno1 antibody

Background

ENO1 is one of three enolase isoenzymes found in mammals, the protein alpha-enolase, a homodimeric soluble enzyme, and is also a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter.

Molecular Weight

47169

NCBI Accession

NP_001188412, NP_001419

UniProt

P06733