FAK antibody (AA 396-423)

Catalog No. ABIN1882121

There are 2+ publications for this product available. The Rabbit Polyclonal anti-FAK antibody is suitable to detect FAK in samples from Human. It has been validated for WB, IF, IHC (p) and FACS.

Quick Overview for FAK antibody (AA 396-423) (ABIN1882121)

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FAK antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Clone: RB20775

Product Details anti-FAK Antibody

Binding Specificity: AA 396-423

Predicted Reactivity: C, M, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This PTK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 396-423 amino acids from the Central region of human PTK2.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. WB: 1:1000. IHC-P: 1:50~100. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Expiry Date: 6 months

References for anti-FAK antibody (ABIN1882121)

Stowe, Turlings, Loughrin, Lewis, Tumlinson: "The chemistry of eavesdropping, alarm, and deceit." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 1, pp. 23-8, (1995) (PubMed).

Calalb, Polte, Hanks: "Tyrosine phosphorylation of focal adhesion kinase at sites in the catalytic domain regulates kinase activity: a role for Src family kinases." in: Molecular and cellular biology, Vol. 15, Issue 2, pp. 954-63, (1995) (PubMed).

Target Details for FAK

Target: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Alternative Name: PTK2

Background: PTK2 is a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. This protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies.

Molecular Weight: 119233

NCBI Accession: NP_001186578, NP_722560

UniProt: Q05397

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling