E2F1 antibody (AA 133-322)

Catalog No. ABIN1886014

This Rabbit Polyclonal antibody specifically detects E2F1 in WB and IF. It exhibits reactivity toward Human.

Quick Overview for E2F1 antibody (AA 133-322) (ABIN1886014)

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E2F1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-E2F1 Antibody

Binding Specificity: AA 133-322

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 133 and 322 (Q01094) of E2F1

Application Details

Application Notes: Suggested dilutions:
Western blotting: 1.500-1.3000
Immunofluorescence: 1.100-1.200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 0.1 M Tris-buffered saline with 10 % Glycerol (pH 7.0).0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: Biohazard Informations: This product contains thimerosal which is hazardous.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for E2F1

Target: E2F1 (E2F Transcription Factor 1 (E2F1))

Alternative Name: E2F1

Background: The protein encoded by this gene is a member of the E2F family of transcription factors.The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.The E2F proteins contain several evolutionally conserved domains found in most members of the family.These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain.This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain.This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner.It can mediate both cell proliferation and p53-dependent/independent apoptosis.[provided by RefSeq]

Molecular Weight: 47 kDa

Gene ID: 1869

NCBI Accession: NM_005225, NP_005216

Pathways: p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy