AIF antibody (AA 517-531)

Catalog No. ABIN1886885

The Rabbit Polyclonal anti-AIF antibody has been validated for WB, IHC and ELISA. It is suitable to detect AIF in samples from Human, Mouse and Rat.

Quick Overview for AIF antibody (AA 517-531) (ABIN1886885)

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-AIF Antibody

Binding Specificity: AA 517-531

Purification: Ion exchange chromatography purified

Immunogen: Peptide corresponding to amino acids 517 to 531 of human AIF.This sequence is identical to those of mouse and rat AIF.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid freezing and thawing repeatly.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for short term use.Store at -20 °C for long term preservation.

Target Details for AIF

Target: AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))

Alternative Name: AIF

Background: Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation.These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins.A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF).Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria.AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9.AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli.AIF is highly conserved between human and mouse and widely expressed.
Synonyms: AIF

Molecular Weight: 67 kDa

UniProt: O95831

Pathways: Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect