Retinoblastoma 1 antibody (pSer249, pTyr252)

Catalog No. ABIN189976

This Rabbit Polyclonal antibody specifically detects Retinoblastoma 1 in WB. It exhibits reactivity toward Human.

Quick Overview for Retinoblastoma 1 antibody (pSer249, pTyr252) (ABIN189976)

Target: Retinoblastoma 1 (RB1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Retinoblastoma 1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Retinoblastoma 1 Antibody

Binding Specificity: pSer249, pTyr252

Purpose: Anti-Retinoblastoma phosphoserine 249/phosphothreonine 252 Rabbit Polyclonal Antibody

Specificity: This antibody recognizes human Rb protein phosphorylated at serine 249 and threonine 252. Reactivity with other species has not been investigated.

Cross-Reactivity: Human

Purification: Affinity - Purified

Immunogen: Phosphopeptide region of human Rb protein containing Serine 249 and threonine 252.

Application Details

Application Notes: Immunoblotting: use at 0.25-1 μg/mL.
End users should determine optimal concentration for their application.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Dilute in PBS or medium which is identical to that used in the assay system.

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.05 % sodium azide, 1 mg/mL BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -80 °C

Storage Comment: Store at -80°C. Prepare appropriate aliquots to avoid multiple freeze-thaw cycles.

Target Details for Retinoblastoma 1

Target: Retinoblastoma 1 (RB1)

Alternative Name: Retinoblastoma-Associated Protein

Background: Retinoblastoma-associated protein,Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity). {UniProtKB:P13405, UniProtKB:P33568, PubMed:10499802, PubMed:15084261}., (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. {PubMed:1316611, PubMed:17974914, PubMed:18701596, PubMed:2839300, PubMed:8892909}.,Nucleus,p105-Rb, p110-RB1, pRb, Rb, pp110

Gene ID: 5925

NCBI Accession: NP_000312

UniProt: P06400

Pathways: Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy