H2AFX antibody (pSer139)

Catalog No. ABIN1900929

This anti-H2AFX antibody is a Rabbit Polyclonal antibody detecting H2AFX in IHC, IF, IHC (p) and ICC. Suitable for Human, Mouse, Cow, Dog, Rabbit, Pig and Guinea Pig.

Quick Overview for H2AFX antibody (pSer139) (ABIN1900929)

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Reactivity: Human, Mouse, Cow, Dog, Rabbit, Pig, Guinea Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This H2AFX antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-H2AFX Antibody

Binding Specificity: pSer139

Specificity: Region surrounding phosphorylated serine 139 of human histone H2AX using the numbering given for Swiss-Prot entry P16104 (GeneID 3014).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:100 - 1:500), IF (1:50 - 1:500), IHC, IHC-P (1:100 - 1:500)

Usage: Immunohistochemistry: Formalin-Fixed Paraffin-Embedded Tissue 1:100 - 1:500: Antigen retrieval is recommended. Antigen retrieval with citrate buffer will enhance staining. Formaldehyde-Fixed Cells 1:100 - 1:500: Permeabilization with Triton-X 100 is recommended. Likely to work with frozen sections. In some cases, the antibody may be diluted further than indicated. Human controls: Breast Carcinoma, Colon Carcinoma, Ovarian Carcinoma. Mouse controls: Squamous Cell Carcinoma.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline, 0.1 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for H2AFX

Target: H2AFX (H2A Histone Family, Member X (H2AFX))

Alternative Name: H2AX

Background: Name/Gene ID: H2AFX

Synonyms: H2AFX, H2AX, Histone H2A.x, H2A.X, H2A histone family, member X, H2AX histone

Gene ID: 3014

Pathways: Telomere Maintenance, DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus