PHLPP2 antibody (C-Term)

Catalog No. ABIN190753

This Goat Polyclonal antibody specifically detects PHLPP2 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PHLPP2 antibody (C-Term) (ABIN190753)

Target: PHLPP2 (PH Domain and Leucine Rich Repeat Protein Phosphatase 2 (PHLPP2))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PHLPP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PHLPP2 Antibody

Binding Specificity: C-Term

Purpose: PHLPP2

Sequence: PHEEDRTEPP EEFD

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-PHEEDRTEPPEEFD, from the C Terminus of the protein sequence according to NP_055835.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 140 kDa band observed in Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysates (calculated MW of 134 kDa according to NP_055835.2). Recommended concentration: 0.1-0.3 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:32000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PHLPP2

Target: PHLPP2 (PH Domain and Leucine Rich Repeat Protein Phosphatase 2 (PHLPP2))

Alternative Name: PHLPPL

Background: PHLPP2, PHLPPL, PH domain and leucine rich repeat protein phosphatase-like , KIAA0931

Gene ID: 23035

NCBI Accession: NP_055835

Pathways: PI3K-Akt Signaling, Fc-epsilon Receptor Signaling Pathway