APH1A antibody (Internal Region)

Catalog No. ABIN190762

Product Details anti-APH1A Antibody

Target: APH1A (Anterior Pharynx Defective 1 Homolog A (C. Elegans) (APH1A))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This APH1A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purpose: APH1A

Sequence: HVTDRSDARL QYG

Specificity: This antibody is expected to recognize isoform 1 (NP_001071096.1) and isoform 2 (NP_057106.2).

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-HVTDRSDARLQYG, from the internal region of the protein sequence according to NP_001071096.1, NP_057106.2.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: Paraffin embedded Human Placenta and Kidney. Recommended concentration: 5 μg/mL.

Western Blot: Approx 26 kDa band observed in Human Brain (Cerebral Cortex) lysates (calculated MW of 26.8 kDa according to NP_057106.2). Recommended concentration: 0.05-0.1 μg/mL. Primary incubation was 1 hour.

Peptide ELISA: antibody detection limit dilution 1:128000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for APH1A

Target: APH1A (Anterior Pharynx Defective 1 Homolog A (C. Elegans) (APH1A))

Alternative Name: APH1A (APH1A Products)

Background: APH1A, anterior pharynx defective 1 homolog A (C. elegans), 6530402N02Rik, APH-1A, CGI-78 , anterior pharynx defective 1 homolog A

Gene ID: 51107

NCBI Accession: NP_001071096, NP_057106

Pathways: Notch Signaling, Neurotrophin Signaling Pathway