DPP4 antibody (Dipeptidyl-Peptidase 4) (Internal Region)

Details for Product anti-DPP4 Antibody No. ABIN190769
Target
Binding Specificity
Internal Region
31
16
16
9
9
7
6
4
4
4
3
3
3
3
3
2
2
2
1
1
1
1
1
1
Reactivity
Rat
176
53
49
6
5
4
3
3
3
3
3
2
2
2
1
1
1
Host
Goat
102
98
18
8
3
Clonality
Polyclonal
Conjugate
This DPP4 antibody is un-conjugated
32
22
18
9
6
5
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
ELISA, Western Blotting (WB)
115
96
65
48
47
41
23
11
9
4
3
2
2
1
1
1
1
Options
Purpose DPP4 / CD26
Immunogen Peptide with sequence C-PPHFDKSKKYP, from the internal region of the protein sequence according to NP_001926.2.
Blocking Peptide Blocking peptide for this product available: ABIN369571
Sequence PPHFDKSKKY P
Isotype IgG
Predicted Reactivity Human, Mouse, Rat
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Grade Verified
Target
Alternative Name DPP4 (DPP4 Antibody Abstract)
Background DPP4, CD26, dipeptidyl-peptidase 4 (CD26, adenosine deaminase complexing protein 2) , ADABP, ADCP2, DPPIV, TP103 , T-cell activation antigen CD26, adenosine deaminase complexing protein 2, dipeptidylpeptidase 4 (CD26, adenosine deaminase complexing protein 2), dipeptidylpeptidase IV, dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
Gene ID 1803
NCBI Accession NP_001926
Pathways Peptide Hormone Metabolism, Regulation of Leukocyte Mediated Immunity
Application Notes

DS WB Results: Approx 110 kDa band observed in Rat Lung lysates (calculated MW of 88.3Da according to NP_001926.2). The observed molecular weight of this glycoprotein corresponds to earlier findings in literature with different antibodies (Herrera et al, J Biol Chem. 2001 Jun 1,276(22):19532-9., PMID: 11278278). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Human and Mouse for this particular batch.

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Minimize freezing and thawing.
Storage -20 °C
Storage Comment Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
Images
Image no. 1 for anti-Dipeptidyl-Peptidase 4 (DPP4) (Internal Region) antibody (ABIN190769) ABIN190769 (0.3µg/ml) staining of Rat Lung lysate (35µg protein in RIPA buffer). Dete...