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DS WB Results: Approx 110 kDa band observed in Rat Lung lysates (calculated MW of 88.3Da according to NP_001926.2). The observed molecular weight of this glycoprotein corresponds to earlier findings in literature with different antibodies (Herrera et al, J Biol Chem. 2001 Jun 1,276(22):19532-9., PMID: 11278278). Recommended concentration: 0.3-1 μg/mL. Primary incubation was 1 hour. Preliminary testing was unsuccessful on Human and Mouse for this particular batch.
Peptide ELISA: antibody detection limit dilution 1:64000.