PAX6 antibody (Internal Region)

Catalog No. ABIN190794

Product Details anti-PAX6 Antibody

Target: PAX6 (Paired Box 6 (PAX6))

Binding Specificity: Internal Region

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PAX6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS)

Purpose: PAX6 (internal)

Sequence: REEKLRNQRR QASN

Specificity: This antibody is expected to recognize all reported isoforms: NP_000271.1, NP_001595.2, NP_001297088.1, NP_001355839.1, NP_001355840.1, NP_001355841.1, NP_001355844.1, NP_001355847.1, NP_001355849.1, NP_001355850.1, NP_001355851.1, NP_001355857.1, NP_0013

Cross-Reactivity: Cow, Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence C-REEKLRNQRRQASN, from the internal region of the protein sequence according to NP_000271.1, NP_001595.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 26 kDa band observed in lysates of cell lines U251 and RAW264.7 and in nuclear lysates of cell lines K562 and HeLa, all successfully blocked by incubation with the immunising peptide (calculated MW of 24.8 kDa according to Human NP_001

Peptide ELISA: antibody detection limit dilution 1:32000.

Comment:

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PAX6

Target: PAX6 (Paired Box 6 (PAX6))

Alternative Name: PAX6 (PAX6 Products)

Synonyms: pax6 antibody, an2 antibody, mgda antibody, wagr antibody, pax-6 antibody, pax6a antibody, xpax6 antibody, XLPAX6 antibody, MGC52531 antibody, PAX-6 antibody, AN antibody, AN2 antibody, D11S812E antibody, MGDA antibody, WAGR antibody, Pax-6 antibody, pax6b antibody, 1500038E17Rik antibody, AEY11 antibody, Dey antibody, Gsfaey11 antibody, Sey antibody, PAXZF-A antibody, Pax6.1 antibody, cb280 antibody, fc20e07 antibody, pax-a antibody, pax[zf-a] antibody, paxzfa antibody, wu:fc20e07 antibody, zfpax-6a antibody, zfpax-6b antibody, paired box 6 antibody, paired box 6 L homeolog antibody, paired box 6 S homeolog antibody, paired box 6a antibody, PAX6 antibody, pax6 antibody, Pax6 antibody, pax6.L antibody, pax6.S antibody, pax6a antibody

Background: PAX6, paired box gene 6 (aniridia, keratitis), AN, AN2, D11S812E, MGC17209, MGDA, WAGR, paired box gene 6, paired box homeotic gene 6 (aniridia, keratitis), paired box homeotic gene-6

Gene ID: 5080, 18508, 25509

NCBI Accession: NP_000271, NP_001595

Pathways: Peptide Hormone Metabolism, Carbohydrate Homeostasis