PCK1 antibody (Internal Region)

Catalog No. ABIN190801

This Goat Polyclonal antibody specifically detects PCK1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for PCK1 antibody (Internal Region) (ABIN190801)

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This PCK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PCK1 Antibody

Binding Specificity: Internal Region

Purpose: PCK1 / PEPCKC (internal region)

Sequence: EKEVEDIEKY LEDQ

Cross-Reactivity: Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EKEVEDIEKYLEDQ, from the internal region (near the C Terminus) of the protein sequence according to NP_002582.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 70 kDa band observed in Human Kidney lysates (calculated MW of 69.2 kDa according to NP_002582.2). Recommended concentration: 0.5-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Alternative Name: PCK1

Background: PCK1, PEPCKC , phosphoenolpyruvate carboxykinase 1 (soluble) , MGC22652, PEPCK1, PEP carboxykinase, cytosolic phosphoenolpyruvate carboxykinase 1, phosphoenolpyruvate carboxylase, phosphopyruvate carboxylase

Gene ID: 5105

NCBI Accession: NP_002582

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis