ERK1 antibody (AA 329-379)

Catalog No. ABIN1911952

This Rabbit Polyclonal antibody specifically detects ERK1 in WB, IHC, IF, IHC (p), IP and PLA. It exhibits reactivity toward Human, Dog and Horse.

Quick Overview for ERK1 antibody (AA 329-379) (ABIN1911952)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA)

Product Details anti-ERK1 Antibody

Binding Specificity: AA 329-379

Specificity: Region between residue 329 and 379 of human extracellular signal-related kinase 1 (mitogen-activated protein kinase 3) using the numbering given in entry NP_002737.2 (GeneID 5595).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IF (1:50 - 1:500), IHC, IHC-P (1:100 - 1:500), IP (2 - 5 μg/mg lysate), PLA (1:200 - 1:4000), WB (1:2000 - 1:10000)

Usage: Immunohistochemistry: Antigen retrieval with citrate buffer  pH 6.0 is recommended for FFPE tissue sections. Human controls: Breast Carcinoma, Colon Carcinoma, Testis.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline, 0.1 % BSA, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3 / ERK1

Background: Name/Gene ID: MAPK3
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3

Gene ID: 5595

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins