EPHA2/3/4 antibody

Catalog No. ABIN1945089

Product Details anti-EPHA2/3/4 Antibody

Target: EPHA2/3/4 (EPH Receptor A2/3/4 (EPHA2/3/4))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPHA2/3/4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Isotype: IgG

Application Details

Application Notes: IF: 1:100. WB: 1:1000. WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C,-20 °C

References for anti-EPHA2/3/4 antibody (ABIN1945089)

Zelinski, Zantek, Stewart, Irizarry, Kinch: "EphA2 overexpression causes tumorigenesis of mammary epithelial cells." in: Cancer research, Vol. 61, Issue 5, pp. 2301-6, (2001) (PubMed).

Miao, Burnett, Kinch, Simon, Wang: "Activation of EphA2 kinase suppresses integrin function and causes focal-adhesion-kinase dephosphorylation." in: Nature cell biology, Vol. 2, Issue 2, pp. 62-9, (2000) (PubMed).

Lindberg, Hunter: "cDNA cloning and characterization of eck, an epithelial cell receptor protein-tyrosine kinase in the eph/elk family of protein kinases." in: Molecular and cellular biology, Vol. 10, Issue 12, pp. 6316-24, (1991) (PubMed).

Target Details for EPHA2/3/4

Target: EPHA2/3/4 (EPH Receptor A2/3/4 (EPHA2/3/4))

Alternative Name: EPHA2/3/4 (EPHA2/3/4 Products)

Background: Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand- independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.

Molecular Weight: 108266 Da

Gene ID: 1969

UniProt: P29317