EPHA2/3/4 antibody

Catalog No. ABIN7214760

This Rabbit Polyclonal antibody specifically detects EPHA2/3/4 in WB, IF and ELISA. It exhibits reactivity toward Human and Rat.

Quick Overview for EPHA2/3/4 antibody (ABIN7214760)

Target: EPHA2/3/4 (EPH Receptor A2/3/4 (EPHA2/3/4))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPHA2/3/4 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA

Product Details anti-EPHA2/3/4 Antibody

Purpose: Rabbit Anti-EphA2/3/4 Polyclonal Antibody

Specificity: EphA2/3/4 Polyclonal Antibody detects endogenous levels of EphA2/3/4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human EphA2/3/4 around the non-phosphorylation site of Y588/596

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IF 1:200-1:1000,ELISA 1:40000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for EPHA2/3/4

Target: EPHA2/3/4 (EPH Receptor A2/3/4 (EPHA2/3/4))

Alternative Name: EphA2/3/4

Background: EPHA2, ECK, Ephrin type-A receptor 2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, EPHA3, ETK, ETK1, HEK, TYRO4, Ephrin type-A receptor 3, EPH-like kinase 4, EK4, hEK4, HEK, Human embryo kinase, Tyrosine-protein kinase TYROEPHA2 belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. EPHA2 encodes a protein that binds ephrin-A ligands. Mutations in EPHA2 are the cause of certain genetically-related cataract disorders.

Molecular Weight: 130kD

Gene ID: 1969, 2042, 2043

UniProt: P29317, P29320, P54764