IGF1R antibody (pTyr1165, pTyr1166)

Catalog No. ABIN196774

This Rabbit Polyclonal antibody specifically detects IGF1R in WB and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for IGF1R antibody (pTyr1165, pTyr1166) (ABIN196774)

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IGF1R antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-IGF1R Antibody

Binding Specificity: pTyr1165, pTyr1166

Specificity: This antibody detects endogenous levels of IGF-1R only when phosphorylated at Tyrosine 1165/1166.

Purification: Immunoaffinity Chromatography

Immunogen: Synthetic phosphopeptide derived from human IGF-1R around the phosphorylation site of Tyrosine 1165/1166 (T-D-YP-YP-R-K).

Application Details

Application Notes: Suitable for use in Western blot (1/500approx. 1/1000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4 containing 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for IGF1R

Target: IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))

Alternative Name: CD221 / IGF1R

Background: There are two kinds of IGF receptors based on their relative affinities for IGF1 versus IGF2. The IGF1 receptor prefers IGF1 over IGF2 and weakly binds insulin. IGF1 receptor is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha (130 kD) and two beta (95 kD) subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The IGF1 receptor is therefore similar in structure to the insulin receptor. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The IGF1 receptor is highly expressed in all cell types and tissues and is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival.Synonyms: IGF-I receptor, IGF1 Receptor, Insulin-like growth factor 1 receptor

Gene ID: 3480

NCBI Accession: NP_000866

UniProt: P08069

Pathways: RTK Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy