PFKM antibody (Biotin)

Catalog No. ABIN1968073

This Goat Polyclonal antibody specifically detects PFKM in WB, ELISA and IF. It exhibits reactivity toward Rabbit.

Quick Overview for PFKM antibody (Biotin) (ABIN1968073)

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Reactivity: Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This PFKM antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF)

Product Details anti-PFKM Antibody (Biotin)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle]. Cross reactivity against Fructose-6-Phosphate Kinase from other sources may occur but have not been specifically determined.

Purification: Ion exchange chromatography

Immunogen: Fructose-6-Phosphate Kinase [Rabbit Muscle].

Type of Immunogen: Purified protein

Application Details

Application Notes: Approved: ELISA (1:4000 - 1:20000), IF, WB (1:500 - 1:2000)

Usage: This purified antibody has been tested for use in ELISA, immunofluorescence microscopy and western blot. Expect a band approximately 48 kD in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Rabbit

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 1 % BSA

Handling Advice: avoid freeze thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

Target Details for PFKM

Target: PFKM (phosphofructokinase, Muscle (PFKM))

Alternative Name: PFKM / PFK-1

Background: Name/Gene ID: PFKM

Synonyms: PFKM, 6-phosphofructo-1-kinase, GSD7, PFK1, PFKA, PFKX, Phosphofructokinase-M, Phosphofructokinase 1, PFK-1, PFK-A, Phosphofructokinase, muscle, Phosphohexokinase

Gene ID: 5213

Pathways: Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Warburg Effect