CDKN1B antibody (Ser10)

Catalog No. ABIN197350

This Rabbit Polyclonal antibody specifically detects CDKN1B in WB and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CDKN1B antibody (Ser10) (ABIN197350)

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDKN1B antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDKN1B Antibody

Binding Specificity: Ser10

Specificity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This antibody detects endogenous levels of total p27Kip1 protein.

Purification: Immunoaffinity Chromatography.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human p27Kip1 around the phosphorylation site of serine 10 (N-G-Sp-P-S).

Application Details

Application Notes: Western blot: 1/500-1/1000. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide as preservative and 50 % Glycerol as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody (in aliquots) at -20 °C.

Target Details for CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Alternative Name: CDKN1B / KIP1

Background: P27 is a cyclin dependent kinase inhibitor (CKI) which controls G1/S phase transition in the cell cycle. p27 prevents cell cycle progression by inhibiting the activity of cyclin E-Cdk2 and cyclin D-Cdk4/6, thus preventing E2F activation and S phase progression. Low p27 levels and/or cytosolic mis-localisation have been associated with the development of lung, breast, colon, oesophagus, and prostate tumours.Synonyms: Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, p27Kip1

Gene ID: 1027

NCBI Accession: NP_004055

UniProt: P46527

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy