MEK1 antibody (Ser217)
Quick Overview for MEK1 antibody (Ser217) (ABIN197430)
Target
See all MEK1 (MAP2K1) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- Ser217
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Specificity
- This antibody AP02714PU detects endogenous levels of total MEK1/MEK2 protein.
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Purification
- Immunoaffinity Chromatography using epitope-specific immunogen.
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Immunogen
- The antiserum was produced against synthesized non-phosphopeptide derived from Human MEK1 around the phosphorylation site of Serine 217 (I-D-SP-M-A).
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anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 2-150) antibody
MAP2K1 Reactivity: Human ELISA, IHC (p), IF (cc), IF (p), FACS, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer298) antibodyKD Validated MAP2K1 Reactivity: Human WB, ICC, FACS Host: Rabbit Monoclonal 24GB285 unconjugated Recombinant Antibody
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 Reactivity: Human, Mouse, Rat, Dog, Monkey WB, IHC, IF, IHC (p) Host: Mouse Monoclonal 3H3 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 Reactivity: Human, Mouse, Rat, Dog, Monkey WB, IHC, IF, IHC (p) Host: Mouse Monoclonal 2H3 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (AA 1-100) antibodyMAP2K1 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyKD Validated MAP2K1 Reactivity: Human WB, FACS Host: Rabbit Monoclonal 23GB2475 unconjugated Recombinant Antibody
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) antibodyMAP2K1 Reactivity: Human, Mouse, Rat, Dog, Monkey WB, IHC, IF, IHC (p) Host: Mouse Monoclonal 3B4 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pSer221) antibodyMAP2K1 Reactivity: Human WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (pThr292) antibodyKD Validated MAP2K1 Reactivity: Human WB, ICC, FACS Host: Rabbit Monoclonal 24GB305 unconjugated Recombinant Antibody
anti-Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1) (N-Term) antibodyMAP2K1 Reactivity: Human, Mouse, Rat, Xenopus laevis WB, IHC, IF, IHC (p) Host: Rabbit Polyclonal unconjugated
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Application Notes
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Western blot: 1/500-1/1000. Immunofluorescence: 1/100-1/200. Immunohistochemistry on Paraffin Sections: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 1.0 mg/mL
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Buffer
- PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % Sodium Azide and 50 % Glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- -20 °C
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Storage Comment
- Store the antibody (in aliquots) at -20 °C.
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- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
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Alternative Name
- MAP2K1
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Background
- Synonyms: Dual specificity mitogen-activated protein kinase kinase 1/2, ERK activator kinase 1, ERK activator kinase 2, MAP kinase kinase 1, MAP kinase kinase 2, MAPK/ERK kinase 1, MAPK/ERK kinase 2, MAPKK1, MEK1, MEK2, PRKMK1, PRKMK2
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Gene ID
- 5604
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NCBI Accession
- NP_002746
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UniProt
- Q02750
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Pathways
- MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
Target
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