CHEK1 antibody (AA 250-300)

Catalog No. ABIN1977789

This anti-CHEK1 antibody is a Rabbit Polyclonal antibody detecting CHEK1 in WB, IHC, IF, ICC, IHC (p), IP and PLA. Suitable for Human, Dog and Pig.

Quick Overview for CHEK1 antibody (AA 250-300) (ABIN1977789)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reactivity: Human, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHEK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Proximity Ligation Assay (PLA)

Product Details anti-CHEK1 Antibody

Binding Specificity: AA 250-300

Specificity: Region between residues 250 and 300 of human Checkpoint Kinase 1 using the numbering given in SwissProt entry O14757 (GeneID 1111).

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:500 - 1:2000), IF (1:500 - 1:2000), IHC, IHC-P (1:1000 - 1:5000), IP (1 - 3 μg/mg lysate), PLA (1:1000 - 1:10000), WB (1:10000 - 1:25000)

Usage: Immunohistochemistry: Antigen retrieval with Tris-EDTA  pH 9.0 is recommended for FFPE tissue sections. Human controls: Colon Carcinoma, HeLa Cells, Testis.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-citrate/phosphate buffer,  pH 7-8, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C for up to 1 year.

Expiry Date: 12 months

Target Details for CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Alternative Name: CHEK1 / CHK1

Background: Name/Gene ID: CHEK1
Subfamily: NIM1
Family: Protein Kinase

Synonyms: CHEK1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase 1, CHK1, Chk1-S, Protein kinase chk1, Checkpoint kinase-1

Gene ID: 1111

Pathways: p53 Signaling, Apoptosis, Cell Division Cycle, DNA Damage Repair