TLR4 antibody

Catalog No. ABIN198155

The Mouse Monoclonal anti-TLR4 antibody is suitable to detect TLR4 in samples from Human and Monkey. It has been validated for FACS, IHC, IP, Neut and IHC (fro).

Quick Overview for TLR4 antibody (ABIN198155)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Neutralization (Neut), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: HTA125

Product Details anti-TLR4 Antibody

Specificity: Reacts preferentially, especially in Flow Cytometry, with human TLR4 (CD284) that is associated with MD-2. This clone is a TLR4 (CD284) function-blocking antibody that is useful for studies on the role of TLR4 (CD284) as a receptor for LPS induced cytokine production by TLR4 bearing cells. The antibody was shown to precipitate TLR4 (100kD).

Purification: Purified

Immunogen: Human TLR4.

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo, IHC, IHC-Fr, IP, Neut

Usage: Suitable for use in Flow Cytometry, Immunoprecipitation, and Neutralization (can be used as antagonist for TLR4, it blocks LPS-induced cytokine production). Note: Before use in biological assays, the product must be sterile-filtered and depending on the concentration to be used, dialyzed against culture medium to remove the sodium azide added.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % BSA, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4

Background: Name/Gene ID: TLR4
Family: Toll-like Receptor

Synonyms: TLR4, CD284, CD284 antigen, Homolog of Drosophila toll, TLR-4, TOLL, ARMD10, Toll-like receptor 4, HToll

Gene ID: 7099

UniProt: O00206

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins