ERK1 antibody (C-Term)

Catalog No. ABIN199417

This Rabbit Polyclonal antibody specifically detects ERK1 in WB, IHC, ELISA and IP. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ERK1 antibody (C-Term) (ABIN199417)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-ERK1 Antibody

Binding Specificity: C-Term

Specificity: Reacts strongly with ERK1 and ERK2. A synthetic peptide, corresponding to a 15 amino acid sequence near the carboxyl terminus (339-353 aa of rat ERK1) was conjugated to carrier protein and used as the immunogen. Reactivity to ERK1 and ERK2 has been confirmed by western blot analysis of human A431 and K562 cells, mouse brain and rat brain. Human, mouse and rat (positive control: human A431 and K562 cells, mouse brain and rat brain cell lysates).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (a.a. 339-353) corresponding to a C-terminal segment of rat ERK1.

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IHC (5 - 10 μg/mL), IP, WB

Usage: Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. ELISA: 0.1-1 μg/mL (to coat: 10 μg/mL). Immunoprecipitation: 2-5 μg. Immunohistochemistry: 5-10 μg/mL. Western blotting: ~1:1000. Positive control: Human A431 and K562 cells, mouse brain and rat brain cell lysates).

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3 / ERK1

Background: Name/Gene ID: MAPK3
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3

Gene ID: 5595

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins