CDKN1B antibody (pThr187)

Catalog No. ABIN199529

The Mouse Monoclonal anti-CDKN1B antibody is suitable to detect CDKN1B in samples from Human, Mouse, Rat and Hamster. It has been validated for WB and ELISA.

Quick Overview for CDKN1B antibody (pThr187) (ABIN199529)

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reactivity: Human, Mouse, Rat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This CDKN1B antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CDKN1B Antibody

Binding Specificity: pThr187

Specificity: Specific for p27 when phosphorylated at Thr187. On Western blots, it identifies the target band at ~27kD. It does not react with unphosphorylated p27. Specificity has been confirmed with Thr187-mutant p27 recombinant protein. Reactivity has been confirmed with p27-transfected 293 cells. Based on sequence homology, this antibody may also react with mouse, rat and hamster.

Purification: Protein A purified

Immunogen: Synthetic peptide encompassing the phosphorylated Thr187 residue of human p27.

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), WB (1 - 3 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. ELISA: 0.1-1 μg/mL. Western Blot: 1-3 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for CDKN1B

Target: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Alternative Name: CDKN1B / p27 Kip1

Background: Name/Gene ID: CDKN1B

Synonyms: CDKN1B, KIP1, MEN1B, P27 Kip1, p27KIP1, MEN4, CDKN4

Gene ID: 1027

UniProt: P46527

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy