PLK1 antibody (AA 402-603)

Catalog No. ABIN199557

This anti-PLK1 antibody is a Mouse Monoclonal antibody detecting PLK1 in WB, ELISA, IF and IP. Suitable for Human, Mouse, Rat and Xenopus laevis.

Quick Overview for PLK1 antibody (AA 402-603) (ABIN199557)

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Reactivity: Human, Mouse, Rat, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-PLK1 Antibody

Binding Specificity: AA 402-603

Specificity: Recognizes the human ~68kD Plk1 protein. Cross-reactivity with a 110kD protein of unknown identity is sometimes observed. Species cross-reactivity: mouse, rat and Xenopus.

Purification: Protein A purified

Immunogen: A His-tagged Plk fusion protein (C-term Plk1), aa402-603 of the human Plk1 protein.

Type of Immunogen: Fusion protein

Isotype: IgG1, IgG2b kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IF (2 - 5 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in Immunofluorescence, ELISA, Western Blot and Immunoprecipitation. Immunofluorescence: 2-5 μg/mL. ELISA: 0.1-1 μg/mL. Western Blot: 1-2 μg/mL. Immunoprecipitation: 5 μg. Positive control: HeLa, A431 and NIH 3T3 cells.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for PLK1

Target: PLK1 (Polo-Like Kinase 1 (PLK1))

Alternative Name: PLK1 / PLK-1

Background: Name/Gene ID: PLK1
Subfamily: Polo
Family: Protein Kinase

Synonyms: PLK1, Polo like kinase, Polo-like kinase (Drosophila), Polo-like kinase 1, STPK13, Polo (Drosophia)-like kinase, PLK, PLK-1

Gene ID: 5347

UniProt: P53350

Pathways: Cell Division Cycle, M Phase