TNF alpha antibody

Catalog No. ABIN199734

The Mouse Monoclonal anti-TNF alpha antibody (ABIN199734) specifically detects TNF alpha in WB, ELISA, ICC and Neut. The antibody is reactive with Human samples.

Quick Overview for TNF alpha antibody (ABIN199734)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Neutralization (Neut)

Product Details anti-TNF alpha Antibody

Specificity: Recognizes recombinant human TNF-a. Cross-reactivity: rhTNF-b, rmTNF-a, rrTNF-a, rpTNF-a, rhTNF RI, rhTNF RII, rmTNF RI and rmTNF RII: >0.05 % .

Purification: Protein G purified

Immunogen: RhTNR-a expressed in E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (4 μg/mL), ICC, Neut, WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry and Neutralization. ELISA: 4 μg/mL as a capture antibody. Use 100 μL/well. Western Blot: ~1-2 μg/mL. The detection limit for rhTNFa is 5 ng/lane under non-reducing and reducing conditions using colorimetric detection. 5-50-fold increased sensitivity can be obtained using ECL. Neutralization of Bioactivity: 0.04-0.08 μg/mL typically neutralizes 50 % of the bioactivity of 0.25 ng/mL of rhTNF-a.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 5 % trehalose.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF Alpha

Background: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gene ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome