TNF alpha antibody

Catalog No. ABIN199738

This Rabbit Polyclonal antibody specifically detects TNF alpha in WB, ELISA, FACS and Neut. It exhibits reactivity toward Human and Monkey.

Quick Overview for TNF alpha antibody (ABIN199738)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Neutralization (Neut)

Product Details anti-TNF alpha Antibody

Specificity: Reacts with human natural and recombinant TNF-alpha as assessed by ELISA. Inhibits the biological activity of human natural and recombinant TNF-alpha as determined with L929 and WEHI cells in a cytotoxicity assay. Cross-reacts with rhesus and cynomolgus natural TNF-alpha and lacks cross-reactivity with human lymphotoxin.

Purification: Purified

Immunogen:

Type of Immunogen: Native protein

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, Flo (1:10), Neut, WB (1:10)

Usage: Suitable for use in ELISA, Western Blot, Flow Cytometry and Neutralization studies. Flow Cytometry: 1:10. Western Blot: 1:10. Neutralization assays: sterile filter before use.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide, 0.1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: TNF Alpha

Background: Name/Gene ID: TNF
Family: TNF

Synonyms: TNF, APC1 protein, Cachectin, DIF, TNF Alpha, Tumor necrosis factor-alpha, TNF-alpha, TNF-a, TNFA, Tumor necrosis factor, TNF, macrophage-derived, TNF, monocyte-derived, TNFSF2

Gene ID: 7124

UniProt: P01375

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome