ENOS antibody

Catalog No. ABIN200816

The Mouse Monoclonal anti-ENOS antibody is suitable to detect ENOS in samples from Cow. It has been validated for WB, ELISA and IP.

Quick Overview for ENOS antibody (ABIN200816)

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Reactivity: Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This ENOS antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-ENOS Antibody

Specificity: Specific for endothelial nitric oxide synthase (eNOS) and does not cross-react with either inducible iNOS or neuronal nNOS. The immunoreactive epitope has not been localized, however, this antibody recognizes an epitope distinct from that recognized by Cat. #LS-C23049. Species cross-reactivity: Bovine, likely to react with other mammalian species including mouse and human eNOS proteins based on sequence homology. Lysates Tested: Bovine aortic endothelial cells, and 293 cells stably expressing recombinant bovine eNOS.

Purification: Protein A purified

Immunogen: Purified bovine recombinant eNOS protein.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IP, WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA, Western Blot and Immunoprecipitation. ELISA: 0.1-1 μg/mL. Western Blot: 1-2 μg/mL. Immunoprecipitation: 5 μg/IP.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ENOS

Target: ENOS (NOS3) (Nitric Oxide Synthase 3 (Endothelial Cell) (NOS3))

Alternative Name: NOS3 / eNOS

Background: Name/Gene ID: NOS3

Synonyms: NOS3, Constitutive NOS, CNOS, Endothelial NOS, ECNOS, NOS type III, NOSIII, EC-NOS, ENOS, Nitric oxide synthase 3

Gene ID: 4846

UniProt: P29474

Pathways: ACE Inhibitor Pathway, Regulation of Systemic Arterial Blood Pressure by Hormones, Cellular Response to Molecule of Bacterial Origin, Myometrial Relaxation and Contraction, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling