GAD65+GAD67 (AA 550-600) antibody

Catalog No. ABIN2017588

Product Details

Target: GAD65+GAD67

Binding Specificity: AA 550-600

Reactivity: Mouse, Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Specificity: Rabbit Anti-GAD65+GAD67 Polyclonal Antibody detects endogenous levels of mouse and rat GAD65 and GAD67 protein. This antibody reacts specifically with GAD65 and GAD67 isoforms. It is predicted to react with human GAD65 and GAD67 according to sequence homology.Positive Control: Mouse brain and rat brain

Cross-Reactivity (Details): Rabbit Anti-GAD65+GAD67 Polyclonal Antibody detects endogenous levels of mouse and rat GAD65 and GAD67 protein. This antibody reacts specifically with GAD65 and GAD67 isoforms. It is predicted to react with human GAD65 and GAD67 according to sequence homology.Positive Control: Mouse brain and rat brain

Purification: Immunoaffinity chromatography

Immunogen: KLH-coupled synthetic peptide within AA 550-600 of human GAD65 (Swiss Prot: Q05329) and GAD67 .

Isotype: IgG1

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Western blot: 0.5-1 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details

Target: GAD65+GAD67

Synonyms: CPSQ1 antibody, GAD antibody, SCP antibody, glutamate decarboxylase 1 antibody, GAD1 antibody

Background: Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of glutamate to gamma-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain. There are two isoforms of GAD, GAD65 (GAD2) and GAD67 (GAD1) (65 and 67 kDa, respectively) encoded by two different genes. The two isoforms have distinct intraneuronal distribution in the brain. There is 64% amino acid identity between the two isoforms. The highest degree of diversity appears to be located at the N-terminus, which in GAD65 is required for targeting the enzyme to GABA-containing secretory vesicles. GAD65 is primarily found in nerve terminals. GAD67 has been thought to be primarily located in the nerve cell body and has been identified as a major autoantigen in type 1 diabetes.Rabbit Anti-GAD65+GAD67 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 550-600 of human GAD65 (Swiss Prot: Q05329) and GAD67 (Swiss Prot: Q99259).