BRAF antibody (AA 754-765)

Catalog No. ABIN205677

This anti-BRAF antibody is a Rabbit Polyclonal antibody detecting BRAF in WB. Suitable for Human, Monkey, Dog and Horse.

Quick Overview for BRAF antibody (AA 754-765) (ABIN205677)

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Reactivity: Human, Monkey, Dog, Horse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRAF antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BRAF Antibody

Binding Specificity: AA 754-765

Specificity: Recognizes full length recombinant human BRaf (plus 6His tag, Mr ~94kD) and B-Raf splice variants in cell lysates (Mr ~68-94kD). Species cross-reactivity: Rat and mouse.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%) Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%) Bat (83%).

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to amino acids 754-765 (C-IQAGGYGAFPVH) of human B-Raf, with an N-terminal cysteine added for conjugation to KLH. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Elephant, Panda, Dog, Horse, Opossum (100%), Mouse, Porcine, Turkey, Rabbit, Chicken, Platypus (92%), Bat (83%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: WB (1 - 2 μg/mL)

Usage: Suitable for use in Western Blot, MAP Kinase Activation and MBP Phosphorylation assays. Western Blot: 1-2 μg/mL detects B-Raf splice variants in RIPA lysates from PC12 cells and the full length purified B-Raf, active enzyme. MAP Kinase Activation and MBP Phosphorylation: 4 μg.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for BRAF

Target: BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))

Alternative Name: BRAF / B-Raf

Background: Name/Gene ID: BRAF
Subfamily: Raf
Family: Protein Kinase

Synonyms: BRAF, 94 kDa B-raf protein, B-raf, BRAF1, B Raf, Proto-oncogene B-Raf, p94, B-RAF1, NS7, ONCOGENE BRAF, RAFB1

Gene ID: 673

UniProt: P15056

Pathways: MAPK Signaling, RTK Signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy