TLR4 antibody

Catalog No. ABIN206338

This Mouse Monoclonal antibody specifically detects TLR4 in WB, FACS and IP. It exhibits reactivity toward Human, Dog, Pig, Guinea Pig and Rhesus Monkey.

Quick Overview for TLR4 antibody (ABIN206338)

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Reactivity: Human, Dog, Pig, Guinea Pig, Rhesus Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This TLR4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Product Details anti-TLR4 Antibody

Specificity: Recognizes the human Toll like receptor 4 (TLR4) cell surface antigen. TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 Molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS. Has been demonstrated to block activation of monocytes with LPS. XXX recommend the use of MCA2061EL for this purpose.

Purification: Protein G purified

Immunogen: Ba/F3 cell line expressing TLR4 (CD284).

Isotype: IgG2a

Application Details

Application Notes: Approved: Flo (1:10 - 1:25), IP, WB

Usage: Flow Cytometry: Use 10 μL of the suggested working dilution to label 10^6 cells or 100 μL whole blood. Method sheets are available on request.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for TLR4

Target: TLR4 (Toll-Like Receptor 4 (TLR4))

Alternative Name: TLR4

Background: Name/Gene ID: TLR4
Family: Toll-like Receptor

Synonyms: TLR4, CD284, CD284 antigen, Homolog of Drosophila toll, TLR-4, TOLL, ARMD10, Toll-like receptor 4, HToll

Gene ID: 7099

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades, Inflammasome, S100 Proteins