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CD22 antibody

CD22 Reactivity: Human, Primate FACS, WB, IP, Inhibition Host: Mouse Monoclonal MEM-01 unconjugated

Catalog No. ABIN206987

Datasheet as PDF

Target See all CD22 Antibodies

CD22 (CD22 Molecule (CD22))
Reactivity
139

56

23

5

3

2

1

1
Human, Primate
Host
89

66

16

1
Mouse
Compatible Secondaries
Clonality
113

57

1
Monoclonal
Conjugate
90

14

13

9

5

3

3

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
This CD22 antibody is un-conjugated
Application
103

74

48

37

35

18

12

12

11

6

5

4

3

3

3

1

1

1

1

1
Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Blocking Antibody (Inhibition)

Specificity

Reacts with CD22 (BL-CAM), a 130 kD type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes, it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes. The antibody MEM-01 cross-blocks the antibody OTH228 that recognizes uniquely epitope "E", it does not cross-block antibodies RFB-4, CLB22/1 and CLB-BLy1.

Purification

Protein A purified

Immunogen

Raji Burkitt's lymphoma cell line.

Type of Immunogen: Cells

Clone

MEM-01

Isotype

IgG1

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CD22 Reactivity: Human FACS, WB, ELISA, IHC, IF, Coat, StM Host: Mouse Monoclonal BLCAM-1795 unconjugated

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CD22 Reactivity: Human FACS, WB, ELISA, IHC, IF, Coat Host: Mouse Monoclonal BLCAM-1796 unconjugated

anti-CD22 Molecule (CD22) antibody
CD22 Reactivity: Human, Non-Human Primate FACS, WB, IP Host: Mouse Monoclonal MEM-01 unconjugated

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CD22 Reactivity: Human, Non-Human Primate FACS Host: Mouse Monoclonal MEM-01 PE

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CD22 Reactivity: Human FACS Host: Mouse Monoclonal IS7 FITC

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CD22 Reactivity: Human FACS, WB, ELISA, IF, IP Host: Rabbit Monoclonal DM13 unconjugated

anti-CD22 Molecule (CD22) antibody
CD22 Reactivity: Human, Mouse, Rat, Monkey, Dog WB, IHC, IHC (p) Host: Mouse Monoclonal 4C3 unconjugated

anti-CD22 Molecule (CD22) (AA 708-847) antibody
CD22 Reactivity: Human WB, IF Host: Rabbit Polyclonal unconjugated

Application Notes

Approved: Flo, Inhb, IP, WB (2 - 4 μg/mL)

Usage: Flow Cytometry: Recommended dilution - 2-4 μg/mL. Western Blotting: Recommended dilution - 2-4 μg/mL, 120 min on vertical incubator. Positive control - RAJI human lymphoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mL cold Lysis b

Not recommended for: IHC-P

Comment

Target Species of Antibody: Human

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

4 °C,-20 °C

Storage Comment

4°C or -20°C, Avoid freeze-thaw cycles.
Target

CD22 (CD22 Molecule (CD22))

Alternative Name

CD22 (CD22 Products)

Background

Name/Gene ID: CD22
Family: Immunoglobulin

Synonyms: CD22, CD22 Molecule, CD22 antigen, SIGLEC2, SIGLEC-2, T-cell surface antigen Leu-14, B-cell receptor CD22, BL-CAM

Gene ID

933

UniProt

P20273