CD22 antibody

Catalog No. ABIN206987

The Mouse Monoclonal anti-CD22 antibody has been validated for FACS, WB, IP and Inhibition. It is suitable to detect CD22 in samples from Human and Primate.

Quick Overview for CD22 antibody (ABIN206987)

Target: CD22 (CD22 Molecule (CD22))

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD22 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Blocking Antibody (Inhibition)

Clone: MEM-01

Product Details anti-CD22 Antibody

Specificity: Reacts with CD22 (BL-CAM), a 130 kD type I transmembrane glycoprotein (immunoglobulin superfamily) expressed in the cytoplasm of pro-B and pre-B lymphocytes, and on the surface of mature and activated B lymphocytes, it is lost on plasma cells, peripheral blood T lymphocytes, granulocytes and monocytes. The antibody MEM-01 cross-blocks the antibody OTH228 that recognizes uniquely epitope "E", it does not cross-block antibodies RFB-4, CLB22/1 and CLB-BLy1.

Purification: Protein A purified

Immunogen: Raji Burkitt's lymphoma cell line.

Type of Immunogen: Cells

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, Inhb, IP, WB (2 - 4 μg/mL)

Usage: Flow Cytometry: Recommended dilution - 2-4 μg/mL. Western Blotting: Recommended dilution - 2-4 μg/mL, 120 min on vertical incubator. Positive control - RAJI human lymphoma cell line. Sample preparation: Resuspend approx. 50 mil. cells in 1 mL cold Lysis b

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CD22

Target: CD22 (CD22 Molecule (CD22))

Alternative Name: CD22

Background: Name/Gene ID: CD22
Family: Immunoglobulin

Synonyms: CD22, CD22 Molecule, CD22 antigen, SIGLEC2, SIGLEC-2, T-cell surface antigen Leu-14, B-cell receptor CD22, BL-CAM

Gene ID: 933

UniProt: P20273