Vimentin antibody

Catalog No. ABIN207044

The Mouse Monoclonal anti-Vimentin antibody is suitable to detect Vimentin in samples from Human. It has been validated for WB, ELISA, FACS, ICC and Inhibition.

Quick Overview for Vimentin antibody (ABIN207044)

Target: Vimentin (VIM)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Blocking Antibody (Inhibition)

Clone: VI-RE-1

Product Details anti-Vimentin Antibody

Specificity: Reacts with human vimentin, a 57 kD intermediate filament protein expressed on a wide variety of mesenchymal and mesodermal cell types.

Purification: Protein A purified

Immunogen: Recombinant human vimentin.

Type of Immunogen: Recombinant protein

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA, Flo (1 - 10 μg/mL), ICC, Inhb, WB (1 - 2 μg/mL)

Usage: Flow Cytometry: Recommended dilution - 1-10 μg/mL. Western Blotting: Recommended dilution - 1-2 μg/mL, overnight in 4 degrees C. Positive control - LEP-19 cell lysate. Negative control - 3T3 mouse cell line. Sample preparation: Resuspend approx. 50 mil. c

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 15 mM sodium azide, approx,  pH 7.4.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: Name/Gene ID: VIM

Synonyms: VIM, Vimentin

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis