ROCK1 antibody (AA 1114-1126)

Catalog No. ABIN207918

This anti-ROCK1 antibody is a Mouse Monoclonal antibody detecting ROCK1 in WB. Suitable for Human, Mouse, Rat, Monkey, Cow, Bat, Dog and Horse.

Quick Overview for ROCK1 antibody (AA 1114-1126) (ABIN207918)

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Reactivity: Human, Mouse, Rat, Monkey, Cow, Bat, Dog, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ROCK1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 154C1465

Product Details anti-ROCK1 Antibody

Binding Specificity: AA 1114-1126

Specificity: This mAb recognizes the cleaved C-terminus of ROCK-1. It does not recognize the full-length protein. Since ROCK-1 is cleaved by caspase-3 during apoptosis, this may be used for detection of apoptosis.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Bovine, Dog, Bat, Horse, Opossum, Platypus (100%) Rabbit (92%) Chicken (85%).

Purification: Protein G purified

Immunogen: This antibody was generated by immunizing mice with a synthetic peptide sequence corresponding to residues 1114-1126 GNLPESRIEGWLS of human ROCK-1. Immunogen peptide sequence is adjacent to the caspase-3 cleavage site of ROCK-1. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Bovine, Dog, Bat, Horse, Opossum, Platypus (100%), Rabbit (92%), Chicken (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: WB (1 - 2 μg/mL)

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.2 % gelatin, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ROCK1

Target: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Alternative Name: Rho Kinase / ROCK1

Background: Name/Gene ID: ROCK1
Subfamily: ROCK
Family: Protein Kinase

Synonyms: ROCK1, p160 Rho-associated kinase, p160ROCK, p160 ROCK-1, p160-ROCK, p160RhoK, Rho-associated kinase, RockI, Rok, Rok alpha, Rho-alpha kinase, p160 rho-kinase, Rho kinase, ROCK-I, Rokalpha

Gene ID: 6093

UniProt: Q13464

Pathways: Microtubule Dynamics, WNT Signaling, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling