IL-6 antibody

Catalog No. ABIN208765

This Rabbit Polyclonal antibody specifically detects IL-6 in WB and Neut. It exhibits reactivity toward Mouse and Rat.

Quick Overview for IL-6 antibody (ABIN208765)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-6 antibody is un-conjugated

Application: Western Blotting (WB), Neutralization (Neut)

Product Details anti-IL-6 Antibody

Specificity: Reacts with natural and recombinant mouse interleukin 6 (IL-6) as assessed by ELISA. The antibody inhibits the biological activity of natural and recombinant mouse IL-6 as determined with the B9 cell bio-assay. The antibody cross-reacts with natural rat IL-6. IL-6 is a pluripotent 20-22 kD cytokine which plays a role in the pathophysiology of severe infection and regulates the immune response, acute phase reaction and hematopoiesis.

Purification: Purified

Immunogen:

Type of Immunogen: Native protein

Isotype: IgG

Application Details

Application Notes: Approved: Neut, WB (1:10)

Usage: Can be used for Neutralization of mouse and rat IL-6 in bio-assays. Furthermore the antibody is useful for Western Blot and immunoassay. Western Blot: 1:10. For neutralization of biological activity dilutions have to be made according to the amounts IL-6 to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide, 0.1 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Alternative Name: IL6

Background: Name/Gene ID: IL6
Family: Hormone

Synonyms: IL6, B-cell stimulatory factor 2, BSF-2, BSF2, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, Interleukin-6, Interferon beta-2, Interleukin BSF-2, IFNB2, B-cell differentiation factor, CDF, HSF, IL-6

Gene ID: 3569

UniProt: P05231

Pathways: TLR Signaling, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagy, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome