MGMT antibody

Catalog No. ABIN209042

The Mouse Monoclonal anti-MGMT antibody has been validated for WB, IHC, FACS and IHC (p). It is suitable to detect MGMT in samples from Human.

Quick Overview for MGMT antibody (ABIN209042)

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MGMT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-MGMT Antibody

Specificity: Recognizes the human MGMT protein. Reactivity is confirmed with human T cell leukemia CEM/CCRF cells.

Purification: Protein A purified

Immunogen: Recombinant human MGMT protein purified from E. coli.

Type of Immunogen: Recombinant protein

Isotype: IgG2b

Application Details

Application Notes: Approved: Flo (5 - 10 μg/mL), IHC, IHC-P, WB (1 - 3 μg/mL)

Usage: Suitable for use in Immunohistochemistry, Western Blot and Flow Cytometry. Western Blot: 1-3 μg/mL. Flow Cytometry: 5-10 μg/mL. Immunohistochemistry: 10 μg/mL. Note: Staining results for immunohistochemical assays with formalin-fixed, paraffin-embedded tissue sections may be greatly enhanced with an antigen retrieval pre-treatment step using citrate buffer,  pH 6.0.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for MGMT

Target: MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))

Alternative Name: MGMT

Background: Name/Gene ID: MGMT

Synonyms: MGMT

Gene ID: 4255

UniProt: P16455

Pathways: DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus