NF-kB p65 antibody
-
- Target See all NF-kB p65 (NFkBP65) Antibodies
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
-
Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- This NF-kB p65 antibody is un-conjugated
-
Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Gel Shift (GS)
- Specificity
- Recognizes an epitope overlapping the nuclear location signal (NLS) of the p65 subunit of the NFkB heterodimer. Thus it selectively binds to the activated form of NFkB.
- Purification
- Protein A purified
- Immunogen
-
Peptide corresponding to human p65 coupled to BSA.
Type of Immunogen: Synthetic peptide - Isotype
- IgG3
- Top Product
- Discover our top product NFkBP65 Primary Antibody
-
-
- Application Notes
-
Approved: ELISA, Flo, GS (1 - 10 μg/mL), ICC, IF, IHC, IHC-Fr (5 - 10 μg/mL), IHC-P (5 - 10 μg/mL), WB (5 - 10 μg/mL)
Usage: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Gel Supershift, Electrophoretic mobility supershift assays (EMSA), Immunohistochemistry and Immunocytochemistry. Immunofluorescence: 1-10 μg/mL. Flow cytometry: fixed cells only (acetone fixed cells). Western Blot: 5-10 μg/mL (ECL). Immunohistochemistry (Frozen, paraffin): 5-10 μg/mL, APAAP. HIER required Citrate buffer, pH 6.0. Supershift assay: 0.5-1 μg/mL. - Comment
-
Target Species of Antibody: Human
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.6, 0.1 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
-
Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.
-
- Target
- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
- Alternative Name
- RELA / NFKB p65 (NFkBP65 Products)
- Synonyms
- C-Rel antibody, NFKB3 antibody, p65 antibody, NFkB antibody, c-rel antibody, zgc:100833 antibody, Xrel2 antibody, Xrel3 antibody, rel antibody, rel-A antibody, rel2 antibody, rel3 antibody, v-rel antibody, xrel antibody, REL proto-oncogene, NF-kB subunit antibody, RELA proto-oncogene, NF-kB subunit antibody, v-rel reticuloendotheliosis viral oncogene homolog A (avian) antibody, v-rel avian reticuloendotheliosis viral oncogene homolog antibody, v-rel avian reticuloendotheliosis viral oncogene homolog L homeolog antibody, REL antibody, RELA antibody, Rela antibody, Rel antibody, rel antibody, rel.L antibody
- Background
-
Name/Gene ID: RELA
Synonyms: RELA, NF-kappa-B p65delta3, NFKB3, p65, Transcription factor p65 - Gene ID
- 5970
- UniProt
- Q04206
- Pathways
- NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins
-