NF-kB p65 antibody

Catalog No. ABIN209098

This Mouse Monoclonal antibody specifically detects NF-kB p65 in WB, IHC, ELISA, IF, FACS, IHC (p), ICC, IHC (fro) and EMSA. It exhibits reactivity toward Human.

Quick Overview for NF-kB p65 antibody (ABIN209098)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NF-kB p65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Electrophoretic Mobility-Shift Assay (EMSA)

Product Details anti-NF-kB p65 Antibody

Specificity: Recognizes an epitope overlapping the nuclear location signal (NLS) of the p65 subunit of the NFkB heterodimer. Thus it selectively binds to the activated form of NFkB.

Purification: Protein A purified

Immunogen: Peptide corresponding to human p65 coupled to BSA.

Type of Immunogen: Synthetic peptide

Isotype: IgG3

Application Details

Application Notes: Approved: ELISA, Flo, GS (1 - 10 μg/mL), ICC, IF, IHC, IHC-Fr (5 - 10 μg/mL), IHC-P (5 - 10 μg/mL), WB (5 - 10 μg/mL)

Usage: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Gel Supershift, Electrophoretic mobility supershift assays (EMSA), Immunohistochemistry and Immunocytochemistry. Immunofluorescence: 1-10 μg/mL. Flow cytometry: fixed cells only (acetone fixed cells). Western Blot: 5-10 μg/mL (ECL). Immunohistochemistry (Frozen, paraffin): 5-10 μg/mL, APAAP. HIER required Citrate buffer,  pH 6.0. Supershift assay: 0.5-1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.6, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: RELA / NFKB p65

Background: Name/Gene ID: RELA

Synonyms: RELA, NF-kappa-B p65delta3, NFKB3, p65, Transcription factor p65

Gene ID: 5970

UniProt: Q04206

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins