NF-kB p65 antibody (AA 297-308)

Catalog No. ABIN209104

This Rabbit Polyclonal antibody specifically detects NF-kB p65 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse, Rat, Monkey, Cow, Dog, Horse, Bat and Hamster.

Quick Overview for NF-kB p65 antibody (AA 297-308) (ABIN209104)

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Reactivity: Human, Mouse, Rat, Monkey, Cow, Dog, Horse, Bat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NF-kB p65 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-NF-kB p65 Antibody

Binding Specificity: AA 297-308

Specificity: Recognizes the nuclear localization sequence (NLS) NLS of human p65. The epitope overlaps the NLS of the p65 subunit of the NFkB heterodimer.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Woolly monkey, Mouse, Rat, Hamster, Elephant, Panda, Porcine, Bovine, Dog, Bat, Horse (100%) Paramecium (83%).

Purification: Immunoaffinity purified

Immunogen: A synthetic peptide, RIEEKRKRTYET, corresponding to the NLS of the human NFkB p65 protein conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Woolly monkey, Mouse, Rat, Hamster, Elephant, Panda, Porcine, Bovine, Dog, Bat, Horse (100%), Paramecium (83%).

Type of Immunogen: Synthetic peptide - KLH conjugated

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:100 - 1:1000), IF (1:200), IHC (1:100), WB (1:1000)

Usage: Suitable for Immunohistochemistry, Immunofluorescence, Western Blot, ELISA and Supershift assays. Western Blot: 1:1000. Immunohistochemistry: 1:100. Immunofluorescence microscopy: 1:100. Super shift: 0.5-1 μL/assay.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for NF-kB p65

Target: NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))

Alternative Name: RELA / NFKB p65

Background: Name/Gene ID: RELA

Synonyms: RELA, NF-kappa-B p65delta3, NFKB3, p65, Transcription factor p65

Gene ID: 5970

UniProt: Q04206

Pathways: NF-kappaB Signaling, RTK Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Toll-Like Receptors Cascades, S100 Proteins