UCHL1 antibody

Catalog No. ABIN210679

This Mouse Monoclonal antibody specifically detects UCHL1 in WB, IHC, ELISA and IHC (p). It exhibits reactivity toward Human and Rat.

Quick Overview for UCHL1 antibody (ABIN210679)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This UCHL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 31A3

Product Details anti-UCHL1 Antibody

Specificity: Recognizes protein gene product 9.5 (PGP9.5), a ubiquitin hydrolase which is widely expressed in neuronal tissues and represents 1-2 % of total soluble brain proteins. PGP9.5, also known as ubiquitin C-terminal hydrolase 1 (UCHL-1), is involved in the regulation of the ubiquitin pathway. Stains neuronal cell bodies and axons in the CNS and periphery, small nerve fibres in peripheral tissues, neuroendocrine cells in the pituitary, thyroid, pancreas and tumors of the DNES. Clones 31A3 and each recognize a different epitope towards the N-terminus of the protein. Also recognizes PGP9.5 in other species, including rat and rabbit but evidence suggests it does not bind to PGP9.5 in guinea pigs. Has been used successfully as a capture reagent with clone 13C4 as a detection reagent in a sandwich ELISA to evaluate contamination of processed meat samples with neuronal tissue.

Purification: Protein G purified

Immunogen: Native, from brain.

Isotype: IgG1

Application Details

Application Notes: Approved: ELISA (1:500 - 1:2000), IHC, IHC-P (1:100 - 1:400), WB

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: UCHL1 / PGP9.5

Background: Name/Gene ID: UCHL1
Subfamily: Cysteine C12
Family: Protease

Synonyms: UCHL1, Ubiquitin C-terminal hydrolase, PARK5, PGP9.5, PGP95, Ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Uch-L1

Gene ID: 7345

UniProt: P09936

Pathways: Feeding Behaviour