ERK1 antibody (AA 324-345)

Catalog No. ABIN210910

This Mouse Monoclonal antibody specifically detects ERK1 in WB, ELISA and IP. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for ERK1 antibody (AA 324-345) (ABIN210910)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-ERK1 Antibody

Binding Specificity: AA 324-345

Specificity: Reacts strongly with both ERK1 and ERK2. Species cross-reactivity: Rat, human, canine and mouse.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide (aa324-345) corresponding to a segment near the C-terminus of rat ERK1.

Type of Immunogen: Synthetic peptide

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (0.1 - 1 μg/mL), IP, WB (1 μg/mL)

Usage: Suitable for use in ELISA, Immunoprecipitation and Western Blot. ELISA: 0.1-1 μg/mL. Immunoprecipitation: 2-5 μg/reaction. Western Blot: 1 μg/mL. Positive control: Human A431 and K562 cells, canine MDCK cells, mouse brain, rat brain and recombinant ERK2.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4, 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: MAPK3 / ERK1

Background: Name/Gene ID: MAPK3
Subfamily: MAPK
Family: Protein Kinase

Synonyms: MAPK3, ERK-1, ERT2, HUMKER1A, HS44KDAP, MAP kinase 1, MAP kinase isoform p44, MAPK 1, MAPK 3, p44, p44MAPK, p44-MAPK, p44ERK1, Insulin-stimulated MAP2 kinase, MAP kinase 3, p44-ERK1, ERK1, PRKM3

Gene ID: 5595

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins