Glucocorticoid Receptor antibody

Catalog No. ABIN211167

Product Details anti-Glucocorticoid Receptor Antibody

Target: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Reactivity: Mouse, Rat, Hamster

Host: Mouse

Clonality: Monoclonal

Conjugate: This Glucocorticoid Receptor antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Specificity: Recognizes glucocorticoid receptor, Mr 85-100kD. Species cross-reactivity: Rat, mouse and hamster. Does not react with human.

Purification: Protein G purified

Immunogen: Native glucocorticoid receptor purified from rat liver.

Type of Immunogen: Purified protein

Isotype: IgG2b

Application Details

Application Notes: Approved: IP, WB

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot Analysis: 1-8 μg/mL of this lot detected Glucocorticoid Receptor in RIPA lysates from L6, PC12, and 3T3/A31 cells. L6 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Glucocorticoid Receptor (4 μg/mL). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates glucocorticoid receptor (~97kD). Immunoprecipitation: This antibody has been reported by an independent laboratory to immunoprecipitate glucocorticoid receptor from WEHI-7 and WCL2 cells.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide, 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Glucocorticoid Receptor

Target: Glucocorticoid Receptor (NR3C1) (Nuclear Receptor Subfamily 3, Group C, Member 1 (Glucocorticoid Receptor) (NR3C1))

Alternative Name: NR3C1/Glucocorticoid Receptor (NR3C1 Products)

Synonyms: GR antibody, gcr antibody, grl antibody, xGR antibody, gccr antibody, gr antibody, zgc:113038 antibody, NR3C1 antibody, GCL antibody, LOC100302444 antibody, Gcr antibody, Grl antibody, Grl-1 antibody, Grl1 antibody, GRL antibody, GCCR antibody, GCR antibody, NO antibody, GR-A antibody, nr3c1 antibody, nuclear receptor subfamily 3 group C member 1 antibody, nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor) antibody, nuclear receptor subfamily 3, group C, member 1 antibody, glucocorticoid receptor antibody, NR3C1 antibody, nr3c1 antibody, LOC100302444 antibody, Nr3c1 antibody

Background: Name/Gene ID: NR3C1
Subfamily: NR3 Steroid receptor
Family: NHR

Synonyms: NR3C1, Glucocorticoid Receptor beta, GR, GCCR, GCR, Glucocorticoid receptor, Glucocorticoid receptor alpha, GRL

Gene ID: 2908

UniProt: P04150

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Regulation of Muscle Cell Differentiation, Regulation of Carbohydrate Metabolic Process