IGF1 antibody

Catalog No. ABIN211952

The Mouse Monoclonal anti-IGF1 antibody has been validated for WB, IHC, IP, Neut and IHC (p). It is suitable to detect IGF1 in samples from Human, Mouse and Rat.

Quick Overview for IGF1 antibody (ABIN211952)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This IGF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Neutralization (Neut), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-IGF1 Antibody

Specificity: Recognizes human IGF1. Cross-reactivity with IGF-II: ~40 % . Species cross-reactivity: mouse, rat and possibly chicken. Occasional difficulty neutralizing rat IGF1 due to binding protein interference.

Purification: Protein G purified

Immunogen: Human insulin-like growth factor I (IGF1) purified from human plasma.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), IP, Neut (10 - 20 μg/mL), WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Neutralization and Immunohistochemistry. Immunoprecipitation: 5 μg immunoprecipitated 100 ng IGF1. Neutralization: 10-20 μg/mL can inhibit the activity of 10 ng/mL of IGF1 as determined by testing with chicken embryo fibroblasts (CEF) using an ATP endpoint assay. Immunohistochemistry: 10 μg/mL. Reported to detect IFG1 in formalin-fixed, paraffin-embedded skin sections.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 200 μL sterile ddH2O

Concentration: Lot specific

Buffer: Supplied as a lyophilized powder from 0.1M Tris-glycine,  pH 7.4, 0.15  mM sodium chloride, 0.1  mM EDTA. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid frequent freeze and thaws.

Storage: -20 °C

Storage Comment: Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid freeze-thaw cycles.

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: IGF-I / IGF1

Background: Name/Gene ID: IGF1
Family: Hormone

Synonyms: IGF1, IGF-IA, IGF-IB, IGFI, IBP1, IGF-I, Insulin-like growth factor 1, Insulin-like growth factor I, Insulin-like growth factor IA, Insulin-like growth factor IB, Mechano growth factor, Somatomedin-C, IGF1A, MGF

Gene ID: 3479

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation