ERBB4 antibody (Internal Region)

Catalog No. ABIN213400

This Rabbit Polyclonal antibody specifically detects ERBB4 in IHC and IHC (p). It exhibits reactivity toward Human, Mouse, Monkey, Bat, Horse and Hamster.

Quick Overview for ERBB4 antibody (Internal Region) (ABIN213400)

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Reactivity: Human, Mouse, Monkey, Bat, Horse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERBB4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ERBB4 Antibody

Binding Specificity: Internal Region

Brand: IHC-plus™

Specificity: Human ERBB4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Bat, Hamster, Panda, Horse (100%) Gorilla, Rat, Pig (95%) Opossum (89%) Platypus (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 19 amino acid peptide from internal region of human ERBB4. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey, Marmoset, Mouse, Bat, Hamster, Panda, Horse (100%), Gorilla, Rat, Pig (95%), Opossum (89%), Platypus (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (15 - 30 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 15-32 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ERBB4

Target: ERBB4 (V-Erb-A erythroblastic Leukemia Viral Oncogene Homolog 4 (Avian) (ERBB4))

Alternative Name: ERBB4 / HER4

Background: Name/Gene ID: ERBB4
Subfamily: EGF Receptor
Family: Protein Kinase

Synonyms: ERBB4, ERBB 4, HER4, p180 erb4, p180erbB4

Gene ID: 2066

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway