KIT antibody (Extracellular Domain)

Catalog No. ABIN213610

This Rabbit Polyclonal antibody specifically detects KIT in IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for KIT antibody (Extracellular Domain) (ABIN213610)

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-KIT Antibody

Binding Specificity: Extracellular Domain

Brand: IHC-plus™

Specificity: Human c-Kit. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Predicted Reactivity: Percent identity with other species by BLAST analysis: Human, Gorilla (100%) Gibbon, Monkey (94%) Marmoset, Mouse, Rat (88%) Hamster, Pig (81%).

Purification: Immunoaffinity purified

Immunogen: Synthetic 16 amino acid peptide from extracellular domain of human c-Kit. Percent identity with other species by BLAST analysis: Human, Gorilla (100%), Gibbon, Monkey (94%), Marmoset, Mouse, Rat (88%), Hamster, Pig (81%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (20 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 20 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, less than 0.1 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.

Expiry Date: 12 months

Target Details for KIT

Target: KIT (Mast/stem Cell Growth Factor Receptor (KIT))

Alternative Name: C-Kit / CD117

Background: Name/Gene ID: KIT
Subfamily: PDGF Receptor
Family: Protein Kinase

Synonyms: KIT, C-Kit, CD117, PBT, Piebald trait, Piebald trait protein, Proto-oncogene c-Kit, SCFR, Soluble KIT variant 1, Tyrosine-protein kinase Kit, p145 c-kit, CD117 antigen

Gene ID: 3815

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Sensory Perception of Sound, Stem Cell Maintenance, Production of Molecular Mediator of Immune Response, Regulation of long-term Neuronal Synaptic Plasticity