PUMA antibody (C-Term)

Catalog No. ABIN213906

This anti-PUMA antibody is a Rabbit Polyclonal antibody detecting PUMA in WB, IHC, ICC and IHC (p). Suitable for Human and Mouse.

Quick Overview for PUMA antibody (C-Term) (ABIN213906)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PUMA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PUMA Antibody

Binding Specificity: C-Term

Brand: IHC-plus™

Specificity: A lower band at approximately 16 kDa was detected in MOLT4 and U937 cells, which may represent the PUMA-b form.

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-P (10 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PUMA

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: PUMA

Background: Name/Gene ID: BBC3
Family: Apoptosis

Synonyms: BBC3, Bcl-2-binding component 3, BCL2 binding component 3, JFY-1, JFY1, PUMA

Gene ID: 27113

UniProt: Q9BXH1

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity