LCK antibody (AA 22-36)

Catalog No. ABIN214226

The Mouse Monoclonal anti-LCK antibody has been validated for WB, IHC, FACS, IP, ICC and IHC (p). It is suitable to detect LCK in samples from Human.

Quick Overview for LCK antibody (AA 22-36) (ABIN214226)

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LCK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: Lck-01

Product Details anti-LCK Antibody

Binding Specificity: AA 22-36

Specificity: Peptide corresponding to a. a. 22-36 in human Lck.

Purification: Affinity purified

Immunogen: Synthetic peptide from human LCK.

Type of Immunogen: Synthetic peptide

Isotype: IgG1

Application Details

Application Notes: Approved: Flo, ICC, IHC, IHC-P (15 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 15 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freezing

Storage: 4 °C

Storage Comment: 4°C, avoid freezing.

Target Details for LCK

Target: LCK (Lymphocyte-Specific Protein tyrosine Kinase (LCK))

Alternative Name: p56lck / LCK

Background: Name/Gene ID: LCK
Subfamily: Src
Family: Protein Kinase

Synonyms: LCK, Lck kinase, LSK, p56-LCK, Pp58lck, Tyrosine-protein kinase Lck, p56lck, Protein YT16, Proto-oncogene Lck, YT16

Gene ID: 3932

UniProt: P06239

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling