CBLC antibody (AA 444-458)

Catalog No. ABIN214306

Product Details anti-CBLC Antibody

Target: CBLC (Cbl proto-oncogene C (CBLC))

Binding Specificity: AA 444-458

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBLC antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunofluorescence (IF)

Brand: IHC-plus™

Specificity: Amino acids 444-458 of Human Cbl-c.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from human CBLC.

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:50000), IF, IHC, IHC-P (5 μg/mL), IP, WB (1:500 - 1:3000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.02 M potassium phosphate, 0.15 M sodium chloride,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for CBLC

Target: CBLC (Cbl proto-oncogene C (CBLC))

Alternative Name: CBLC (CBLC Products)

Synonyms: CBL-3 antibody, CBL-SL antibody, RNF57 antibody, Cbl proto-oncogene C antibody, CBLC antibody, Cblc antibody

Background: Name/Gene ID: CBLC

Synonyms: CBLC, CBL-3, CBL3, RNF57, SH3-binding protein CBL-C, SH3-binding protein CBL-3, CBL-c, CBL-SL, RING finger protein 57

Gene ID: 23624

UniProt: Q9ULV8

Pathways: EGFR Signaling Pathway