CD45RO antibody (PE-Cy5)

Catalog No. ABIN2144389

Product Details anti-CD45RO Antibody (PE-Cy5)

Target: CD45RO

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD45RO antibody is conjugated to PE-Cy5

Application: Flow Cytometry (FACS)

Characteristics: 4AUCHL1 reacts with an isoform of CD45, a 180 kDa molecule clustered as CD45RO.

Clone: 4AUCAL-1

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Assay Procedure:

• Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mLtube,
• Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light,
• Add 2 ml1×RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10×,Cat.: FXP001),
• Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 2 mLPBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant,
• Add 0.5 mLPBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).
• [PBS wash buffer: PBS +1 % FBS +0.1 % NaN3, Cat.: FXP005]
• [Cell fixation: 2 % formaldehyde solution]

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Phosphate-buffered solution, pH 7.4, containing and 0.2 % (w/v) BSA

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD45RO

Target: CD45RO

Alternative Name: CD45RO (CD45RO Products)

Background: CD45RO is expressed on memory/activated T cells and cortical thymocytes, in comparison with CD45RA, which is expressed on naive/resting T cells and on medullart thymocytes. CD45RO and CD45RA molecules are important to discriminate naive and memory T cells in the study of the immune system.