PAK4 antibody (Internal Region)

Catalog No. ABIN214461

The Rabbit Polyclonal anti-PAK4 antibody (ABIN214461) specifically detects PAK4 in WB, IHC and IHC (p). The antibody is reactive with Human samples.

Quick Overview for PAK4 antibody (Internal Region) (ABIN214461)

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PAK4 Antibody

Binding Specificity: Internal Region

Specificity: PAK4 antibody is predicted to not cross-react with other PAK family proteins.

Purification: Immunoaffinity purified

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (1 - 2 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 2.5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for 3 months and -20°C, stable for up to 1 year. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for PAK4

Target: PAK4 (P21-Activated Kinase 4 (PAK4))

Alternative Name: PAK4

Background: Name/Gene ID: PAK4
Subfamily: PAK (STE20)
Family: Protein Kinase

Synonyms: PAK4, KIAA1142, p21(CDKN1A)-activated kinase 4, PAK-4, p21-activated kinase 4

Gene ID: 10298

UniProt: O96013

Pathways: RTK Signaling