FOXE3 antibody (AA 235-284)

Catalog No. ABIN6736380

Product Details anti-FOXE3 Antibody

Target: FOXE3 (Forkhead Box E3 (FOXE3))

Binding Specificity: AA 235-284

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXE3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human FOXE3

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa235-284 of human FOXE3 (Q13461, NP_036318). Percent identity by BLAST analysis: Human (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: After adding water, will consist of PBS buffer with 2 % sucrose

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for FOXE3

Target: FOXE3 (Forkhead Box E3 (FOXE3))

Alternative Name: FOXE3 (FOXE3 Products)

Synonyms: FOXE3 antibody, FKHL12 antibody, FREAC8 antibody, dyl antibody, rct antibody, FREAC-8 antibody, HFH7 antibody, HNF3 antibody, foxe4 antibody, lens1 antibody, lens1-A antibody, forkhead box E3 antibody, forkhead box E3 L homeolog antibody, foxe3 antibody, FOXE3 antibody, LOC100301611 antibody, Foxe3 antibody, foxe3.L antibody

Background: Name/Gene ID: FOXE3
Family: Transcription factor

Synonyms: FOXE3, ASMD, FKHL12, Forkhead box E3, Forkhead box protein E3, Forkhead protein 8, FREAC-8, FREAC8

Gene ID: 2301

NCBI Accession: NP_036318

UniProt: Q13461