ATP1A1 antibody

Catalog No. ABIN215644

Product Details anti-ATP1A1 Antibody

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Reactivity: Human, Rat, Mouse, Pig, Dog, Monkey, Xenopus laevis

Host: Mouse

Clonality: Monoclonal

Conjugate: This ATP1A1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificity: Recognizes rabbit Na+/K+ ATPase alpha-1 subunit. Species cross-reactivity: Human, rabbit, monkey, rat, mouse, lamb, canine, porcine and Xenopus.

Purification: Protein G purified

Immunogen: Purified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.

Type of Immunogen: Purified protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IHC, IP, WB (0.05 - 0.1 μg/mL)

Usage: Western Blot: 0.05-0.1 μg/mL detected the a-1 subunit in 20 μg of rat brain microsomal preparations using chemiluminescence. To reduce background, wash the blot with 0.05 % Tween-20 in PBS for 15 minutes.Immunohistochemistry: 1-10 μg/mL on ice-cold methanol-fixed tissue.Immunoprecipitation: 2 μg immunoprecipitated the a-1 subunit from rat brain microsomal preparations.Optimal dilutions to be determined by the researcher.

Comment:

Target Species of Antibody: Rabbit

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 0.1M Tris-Glycine,  pH 7.4, 0.15M sodium chloride, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for ATP1A1

Target: ATP1A1 (Sodium Potassium ATPase, alpha1 (ATP1A1))

Alternative Name: ATP1A1 (ATP1A1 Products)

Synonyms: Atpa-1 antibody, BC010319 antibody, Nkaa1b antibody, K-ATPase antibody, Na+K+ATPase antibody, atp1a1 antibody, atp1a1a.1 antibody, atp1a1l1 antibody, atp[a]1B1 antibody, cb70 antibody, wu:fa03c03 antibody, wu:fb92h10 antibody, atp1a1l5 antibody, atp[a]1B2 antibody, cb707 antibody, wu:fj34g01 antibody, zgc:92351 antibody, ATPase Na+/K+ transporting subunit alpha 1 antibody, ATPase, Na+/K+ transporting, alpha 1 polypeptide antibody, ATPase Na+/K+ transporting subunit alpha 1 L homeolog antibody, ATPase, Na+/K+ transporting, alpha 1a polypeptide, tandem duplicate 1 antibody, ATPase, Na+/K+ transporting, alpha 1b polypeptide antibody, ATP1A1 antibody, Atp1a1 antibody, atp1a1.L antibody, atp1a1a.1 antibody, atp1a1b antibody

Background: Name/Gene ID: ATP1A1
Subfamily: ATPase - P type, type IIC
Family: Transporter

Synonyms: ATP1A1, Na+, K+ ATPase alpha subunit, Na,K-ATPase alpha-1 subunit, Na+/K+ ATPase 1, Sodium pump 1, Sodium pump subunit alpha-1

Gene ID: 476

UniProt: P05023

Pathways: Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Proton Transport, Ribonucleoside Biosynthetic Process