BRIP1 antibody (pSer990) (HRP)

Catalog No. ABIN2170685

The Rabbit Polyclonal anti-BRIP1 antibody has been validated for WB, ELISA, IHC (p) and IHC (fro). It is suitable to detect BRIP1 in samples from Human and Mouse.

Quick Overview for BRIP1 antibody (pSer990) (HRP) (ABIN2170685)

Target: BRIP1 (BRCA1 Interacting Protein C-terminal Helicase 1 (BRIP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRIP1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-BRIP1 Antibody (HRP)

Binding Specificity: pSer990

Specificity: This phosphorylation site is homologous to that of Ser921 in Bovine.

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Cow,Sheep

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human BRIP1 around the phosphorylation site of Ser990

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for BRIP1

Target: BRIP1 (BRCA1 Interacting Protein C-terminal Helicase 1 (BRIP1))

Alternative Name: BRIP1

Background:

Synonyms: OF, BACH1, FANCJ, Fanconi anemia group J protein, Protein FACJ, ATP-dependent RNA helicase BRIP1, BRCA1-associated C-terminal helicase 1, BRCA1-interacting protein C-terminal helicase 1, BRCA1-interacting protein 1, BRIP1

Background: DNA-dependent ATPase and 5' to 3' DNA helicase required for the maintenance of chromosomal stability. Acts late in the Fanconi anemia pathway, after FANCD2 ubiquitination. Involved in the repair of DNA double-strand breaks by homologous recombination in a manner that depends on its association with BRCA1.

Gene ID: 83990

UniProt: Q9BX63

Pathways: DNA Damage Repair